A total of 30 landmarks are placed on the virtually extracted cranial endocast of the extant Two-toed sloth, *Choloepus hoffmanni* (UF-M-25993) and used to capture endocranial shape information. Of those landmarks, 18 are placed on the **dorsal and left lateral** surfaces of the cranial endocast as annotated here. See ventral landmarks 17 - 26 & 29 - 30 here: https://sketchfab.com/3d-models/two-toed-sloth-endocranial-ventral-landmarks-57f6d74d990847fdace8f23f4953fb79. The skull of this specimen is housed in the Mammalogy Collection at the Florida Museum of Natural History, University of Florida.